Descubra tudo sobre as novas possibilidades para adicionar aos seus primers

MBQ-1 e MBQ-2: License-Free Quenchers: a escolha perfeita para as dual-labelled probes de DNA/RNA.

Com MBQ-1 e o MBQ-2, pode melhorar a sua pesquisa com os dark quenchers de alto desempenho que absorvem energia sem emitir fluorescência.

Estamos ansiosos por contribuir com a sua pesquisa através de soluções inovadoras e económicas.

Pedido do Primers Oligos

METABION BLACK QUENCHERS: MBQ-1 AND MBQ-2

MBQ-1 and MBQ-2 stand for metabion Black Quencher 1 and metabion Black Quencher 2.

MBQ-modifications consist of dark quenchers often used in dual-labelled probes for real-time PCR and are structurally identical to their respective BHQ-counterparts.

Dark Quenchers absorb energy without emitting fluorescence. They are in fact non-fluorescent chromophores.

The MBQ-1 (Figure 1) dark quencher exhibits strong absorption between 480 nm and 580 nm, making it highly effective at quenching fluorophores that fluoresce within this range, such as 6-Fam, TET, JOE, HEX, or a variety of Atto dyes.

Figure 1. Structure of 3′-MBQ-1.

The absorbance spectra of MBQ-1 is identical to the absorbance spectra of BHQ-1 (Figure 2), which indicates that also the quenching range is identical.

MBQ-1 can quench fluorescence through both FRET and static quenching mechanisms.

Figure 2. Absorbance spectra of MBQ-1- and BHQ-1-labelled oligonucleotides. As visible, the absorbance max of MBQ-1 is 534 nm, exactly as its counterpart BHQ-1.

The MBQ-2 (Figure 3) dark quencher exhibits strong absorption between 550 nm and 650 nm, making it highly effective at quenching fluorophores that fluoresce within this range, such as Tamra, ROX, or a variety of Atto dyes.

Figure 3. Structure of 3′-MBQ-2.

The absorbance spectra of MBQ-2 is identical to the absorbance spectra of BHQ-2 (Figure 4), which indicates that also the quenching range is identical.

MBQ-2 can quench fluorescence through both FRET and static quenching mechanisms.

Figure 4. Absorbance spectra of MBQ-2- and BHQ-2-labelled oligonucleotides. As visible, the absorbance max of MBQ-2 is 579 nm, exactly as its counterpart BHQ-2.

Metabion offers MBQ-1 and MBQ-2 at 3′ in its DNA and RNA dual-labelled probes portfolio: