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MBQ-1 e MBQ-2: License-Free Quenchers: a escolha perfeita para as dual-labelled probes de DNA/RNA.
Com MBQ-1 e o MBQ-2, pode melhorar a sua pesquisa com os dark quenchers de alto desempenho que absorvem energia sem emitir fluorescência.
Estamos ansiosos por contribuir com a sua pesquisa através de soluções inovadoras e económicas.
Melhoramos o nosso formulário para que seja ainda mais fácil e rápido requisitar primers personalizados e adaptados às suas necessidades.
Pedido do Primers Oligos
Conheça as vantagens e descontos dos nossos Pack Primers
Todas as encomendas estão isentas de portes de envio.
PACK 1000
- 0,30€/base
- Escala 0,02 μmol
- Purificação Standard
- Primers Liofilizados
- Sem Modificações
PACK 500
- 0,36€/base
- Escala 0,02 μmol
- Purificação Standard
- Primers Liofilizados
- Sem Modificações
Qualquer modificação terá um valor acrescido. Consulte-nos para mais informações.
Aos preços apresentados acresce o IVA à taxa em vigor.
Cancelamentos ou alterações:
Lembramos que apenas podemos aceitar o cancelamento ou alteração da encomenda até 30 minutos após o envio da mesma. Após esse período, se a síntese já tiver sido iniciada, poderemos ter de cobrar algum custo.
METABION BLACK QUENCHERS: MBQ-1 AND MBQ-2
MBQ-1 and MBQ-2 stand for metabion Black Quencher 1 and metabion Black Quencher 2.
MBQ-modifications consist of dark quenchers often used in dual-labelled probes for real-time PCR and are structurally identical to their respective BHQ-counterparts.
Dark Quenchers absorb energy without emitting fluorescence. They are in fact non-fluorescent chromophores.
The MBQ-1 (Figure 1) dark quencher exhibits strong absorption between 480 nm and 580 nm, making it highly effective at quenching fluorophores that fluoresce within this range, such as 6-Fam, TET, JOE, HEX, or a variety of Atto dyes.
Figure 1. Structure of 3′-MBQ-1.
The absorbance spectra of MBQ-1 is identical to the absorbance spectra of BHQ-1 (Figure 2), which indicates that also the quenching range is identical.
MBQ-1 can quench fluorescence through both FRET and static quenching mechanisms.
Figure 2. Absorbance spectra of MBQ-1- and BHQ-1-labelled oligonucleotides. As visible, the absorbance max of MBQ-1 is 534 nm, exactly as its counterpart BHQ-1.
The MBQ-2 (Figure 3) dark quencher exhibits strong absorption between 550 nm and 650 nm, making it highly effective at quenching fluorophores that fluoresce within this range, such as Tamra, ROX, or a variety of Atto dyes.
Figure 3. Structure of 3′-MBQ-2.
The absorbance spectra of MBQ-2 is identical to the absorbance spectra of BHQ-2 (Figure 4), which indicates that also the quenching range is identical.
MBQ-2 can quench fluorescence through both FRET and static quenching mechanisms.
Figure 4. Absorbance spectra of MBQ-2- and BHQ-2-labelled oligonucleotides. As visible, the absorbance max of MBQ-2 is 579 nm, exactly as its counterpart BHQ-2.
Metabion offers MBQ-1 and MBQ-2 at 3′ in its DNA and RNA dual-labelled probes portfolio:
–
MBQ-1 as a 3′ modification
BHQ®-1 as a 3′ modification
MW
554.53 g/mol
554.53 g/mol
Absorption range
480-580 nm
480-580 nm
Absorption maximum
534 nm
534 nm
Extinction coefficient (260nm)
8 mM⁻¹cm⁻¹
8 mM⁻¹cm⁻¹
–
MBQ-2 as a 3′ modification
BHQ®-2 as a 3′ modification
MW
556.46 g/mol
556.46 g/mol
Absorption range
550-650 nm
550-650 nm
Absorption maximum
579 nm
579 nm
Extinction coefficient (260nm)
8 mM⁻¹cm⁻¹
8 mM⁻¹cm⁻¹
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